Automatic Correction of Non-uniform Illumination in Transmission Electron Microscopy Images
نویسندگان
چکیده
Transmission electron microscopy (TEM) provides resolutions on the order of a nanometer. Hence, it is a critical imaging modality for biomedical analysis at the cellular level. One of the problems associated with TEM images is variations in brightness due to electron imaging defects or nonuniform support films and specimen staining. These variations render image processing operations such as segmentation more difficult. The correction requires estimation of the global illumination field. In this paper, we propose an automatic method for estimating the illumination field using only image intensity gradients. The closed-form solution is very fast to compute. Automatic Correction of Non-uniform Illumination in Transmission Electron Microscopy Images Tolga Tasdizen Ross Whitaker Robert Marc Bryan Jones
منابع مشابه
Non-uniform Illumination Correction in Transmission Electron Microscopy
Transmission electron microscopy (TEM) provides resolutions on the order of a nanometer. Hence, it is a critical imaging modality for biomedical analysis at the sub-cellular level. One of the problems associated with TEM images is variations in brightness due to electron imaging defects or non-uniform support films and specimen staining. These variations render image processing operations such ...
متن کاملAttenuation correction in myocardial perfusion SPECT using sequential transmission - emission scanning with 99mTc [Persain]
Introduction: Nowadays, Imaging of the myocardial perfusion (MPI) using the single photon emission tomography (SPET) in the diagnosis of coronary artery disease, especially myocardial ischemia, is of great importance. In contrast to the coronary artery angiography, MPI is non-invasive, less expensive and more physiological. Unfortunately, this image is affected by the some artifacts. Thes...
متن کاملLow-dose aberration corrected cryo-electron microscopy of organic specimens.
Spherical aberration (C(s)) correction in the transmission electron microscope has enabled sub-angstrom resolution imaging of inorganic materials. To achieve similar resolution for radiation-sensitive organic materials requires the microscope to be operated under hybrid conditions: low electron dose illumination of the specimen at liquid nitrogen temperature and low defocus values. Initial imag...
متن کاملFully automatic stitching and distortion correction of transmission electron microscope images.
In electron microscopy, a large field of view is commonly captured by taking several images of a sample region and then by stitching these images together. Non-linear lens distortions induced by the electromagnetic lenses of the microscope render a seamless stitching with linear transformations impossible. This problem is aggravated by large CCD cameras, as they are commonly in use nowadays. We...
متن کاملImage Quality Enhancement Using Pixel Wise Gamma Correction
This paper presents a new automatic image enhancement method by modifying the gamma value of its individual pixels. Most of existing gamma correction methods apply a uniform gamma value across the image. Considering the fact that gamma variation for a single image is actually nonlinear, the proposed method locally estimates the gamma values in an image using support vector machine. First, a dat...
متن کامل